charles gersbach (Addgene inc)
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charles gersbach
Charles Gersbach, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna+dcas9+p300+core/pcDNA-dCas9-p300+Core+(Plasmid+%2361357)/pmc13049930-389-31-33
Average 93 stars, based on 79 article reviews
Charles Gersbach, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna+dcas9+p300+core/pcDNA-dCas9-p300+Core+(Plasmid+%2361357)/pmc13049930-389-31-33
Average 93 stars, based on 79 article reviews
charles gersbach - by Bioz Stars,
2026-10
93/100 stars
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shRNA:Article Title: HEXIM1/P-TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization. Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Article Title: HEXIM1/P–TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Construct:Article Title: HEXIM1/P-TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization. Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Article Title: HEXIM1/P–TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Generated:Article Title: HEXIM1/P-TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization. Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Article Title: Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus Article Snippet: sgRNAs targeting promoters of genes of interest (A3A, A3B, A3G, AID) were designed using the open-access web tool Broad Institute Genetic Perturbation Platform. .. PCR products containing the U6 promoter and sgRNA specific for every promoter region were synthesized by two-step mutagenic PCR using Q5 polymerase and purified using a Qiagen gel extraction kit.52,53 The following plasmids were used: Article Title: HEXIM1/P–TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Polymerase Chain Reaction:Article Title: HEXIM1/P-TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization. Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Article Title: Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus Article Snippet: sgRNAs targeting promoters of genes of interest (A3A, A3B, A3G, AID) were designed using the open-access web tool Broad Institute Genetic Perturbation Platform. .. PCR products containing the U6 promoter and sgRNA specific for every promoter region were synthesized by two-step mutagenic PCR using Q5 polymerase and purified using a Qiagen gel extraction kit.52,53 The following plasmids were used: Article Title: HEXIM1/P–TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Amplification:Article Title: HEXIM1/P-TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization. Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Article Title: HEXIM1/P–TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Clone Assay:Article Title: HEXIM1/P-TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization. Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Article Title: Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus Article Snippet: sgRNAs targeting promoters of genes of interest (A3A, A3B, A3G, AID) were designed using the open-access web tool Broad Institute Genetic Perturbation Platform. .. PCR products containing the U6 promoter and sgRNA specific for every promoter region were synthesized by two-step mutagenic PCR using Q5 polymerase and purified using a Qiagen gel extraction kit.52,53 The following plasmids were used: Article Title: HEXIM1/P–TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Bioprocessing:Article Title: HEXIM1/P-TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization. Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Article Title: HEXIM1/P–TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Plasmid Preparation:Article Title: HEXIM1/P-TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization. Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Article Title: Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus Article Snippet: sgRNAs targeting promoters of genes of interest (A3A, A3B, A3G, AID) were designed using the open-access web tool Broad Institute Genetic Perturbation Platform. .. PCR products containing the U6 promoter and sgRNA specific for every promoter region were synthesized by two-step mutagenic PCR using Q5 polymerase and purified using a Qiagen gel extraction kit.52,53 The following plasmids were used: Article Title: Supporting Information Chemical control of a CRISPR-Cas9 acetyltransferase Article Snippet: .. Converting Article Title: Supporting Information Chemical control of a CRISPR-Cas9 acetyltransferase Article Snippet: .. General procedures6: pcDNA-dCas9-VP64 (Addgene plasmid # 47107), pcDNA-dCas9-HA (Addgene plasmid # 61355), Article Title: HEXIM1/P–TEFb complex controls RNA polymerase II pause release and immediate early gene induction following neuronal depolarization Article Snippet: AAV-shRNA-ctrl was a gift from Hongjun Song (Addgene plasmid # 85741). .. The region containing the U6 promoter and shRNA were deleted from constructs. sgRNA were generated as gBLOCKs, PCR amplified, and cloned into the AAV-shRNA-ctrl backbone. Article Title: Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems Article Snippet: The Human Calabrese CRISPR activation pooled library set A was a gift from David Root and John Doench (Addgene #92379). pXPR_502 was a gift from John Doench & David Root (Addgene plasmid # 96923; http://n2t.net/addgene:96923 ; RRID:Addgene_96923). .. In pXPR_502, the sgRNA tetraloop is modified by two PP7 and two MS2 aptamers. pLX-sgRNA was a gift from Eric Lander & David Sabatini (Addgene plasmid # 50662; http://n2t.net/addgene:50662 ; RRID:Addgene_50662). lentiGuide-Puro was a gift from Feng Zhang (Addgene plasmid # 52963). Synthesized:Article Title: Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus Article Snippet: sgRNAs targeting promoters of genes of interest (A3A, A3B, A3G, AID) were designed using the open-access web tool Broad Institute Genetic Perturbation Platform. .. PCR products containing the U6 promoter and sgRNA specific for every promoter region were synthesized by two-step mutagenic PCR using Q5 polymerase and purified using a Qiagen gel extraction kit.52,53 The following plasmids were used: Purification:Article Title: Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus Article Snippet: sgRNAs targeting promoters of genes of interest (A3A, A3B, A3G, AID) were designed using the open-access web tool Broad Institute Genetic Perturbation Platform. .. PCR products containing the U6 promoter and sgRNA specific for every promoter region were synthesized by two-step mutagenic PCR using Q5 polymerase and purified using a Qiagen gel extraction kit.52,53 The following plasmids were used: Gel Extraction:Article Title: Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus Article Snippet: sgRNAs targeting promoters of genes of interest (A3A, A3B, A3G, AID) were designed using the open-access web tool Broad Institute Genetic Perturbation Platform. .. PCR products containing the U6 promoter and sgRNA specific for every promoter region were synthesized by two-step mutagenic PCR using Q5 polymerase and purified using a Qiagen gel extraction kit.52,53 The following plasmids were used: Cloning:Article Title: Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus Article Snippet: sgRNAs targeting promoters of genes of interest (A3A, A3B, A3G, AID) were designed using the open-access web tool Broad Institute Genetic Perturbation Platform. .. PCR products containing the U6 promoter and sgRNA specific for every promoter region were synthesized by two-step mutagenic PCR using Q5 polymerase and purified using a Qiagen gel extraction kit.52,53 The following plasmids were used: Produced:Article Title: Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus Article Snippet: sgRNAs targeting promoters of genes of interest (A3A, A3B, A3G, AID) were designed using the open-access web tool Broad Institute Genetic Perturbation Platform. .. PCR products containing the U6 promoter and sgRNA specific for every promoter region were synthesized by two-step mutagenic PCR using Q5 polymerase and purified using a Qiagen gel extraction kit.52,53 The following plasmids were used: Modification:Article Title: Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems Article Snippet: The Human Calabrese CRISPR activation pooled library set A was a gift from David Root and John Doench (Addgene #92379). pXPR_502 was a gift from John Doench & David Root (Addgene plasmid # 96923; http://n2t.net/addgene:96923 ; RRID:Addgene_96923). .. In pXPR_502, the sgRNA tetraloop is modified by two PP7 and two MS2 aptamers. pLX-sgRNA was a gift from Eric Lander & David Sabatini (Addgene plasmid # 50662; http://n2t.net/addgene:50662 ; RRID:Addgene_50662). lentiGuide-Puro was a gift from Feng Zhang (Addgene plasmid # 52963). |